Set of genetic elements, vector, host cell, set for the production of a nucleic acid sequence, method for in vivo or in vitro production of a nucleic acid sequence, cdna transcription, inhibitor nucleic acid molecule, mrna transcription, heteroduplex molecule and pharmaceutical composition

摘要

"SET OF GENETIC ELEMENTS, VECTOR, PHONE CELLS, SET FOR THE PRODUCTION OF A NUCLEIC ACID HUMIDITY, METHOD FOR IN VIVO OR IN VITRO NUCLEIC ACID, KNOWLEDGE, KNOWLEDGE, KNOWLEDGE, KNOWLEDGE TRANSACTION, PHARMACEUTICAL COMPOSITION ". The present invention relates to methods and compositions for the production of single-stranded cDNA (ss-cDNA) with a cell-based eukaryotic vector system. The vector contains all signaling instructions and enzymatic functions to allow the host cell to produce oss-cDNA encoding a desired nucleic acid sequence (a "sequence of interest"). The components included in the vector for the synthesis of ss-cDNA in vivo are described. Osmesms include (1) a reverse transcriptase gene; (2) a genetic element which provides the template for the desired ssDNA interest sequence; and (3) a second genetic element located next to the genetic element encoding the sequence of interest which provides the primary binding site for reverse transcription by the reverse transcriptase molecule. The vector also contains appropriate promoter (s) / enhancer (s). Also described here is a method for constructing a vector including these components.