Nanoparticle Labels/Electrochemical Immunosensor for Detection of Biomarkers

摘要

A sensitive electrochemical immunosensor based on poly(guanine) functionalized silica nanoparticle label has been developed for the detection of protein biomarker, recombinant human tumor necrosis factor-α(TNF-α).This method is simple, selective, and reproducible for trace biomarker analysis. A remarkable LOD has been achieved through dual signal amplification by poly(Guanine) functionalized silica NPs and catalytic guanine oxidation. The work demonstrates the feasibility of developing an inexpensive, sensitive, and portable device for multiplexed diagnoses of different protein biomarkers.