A Single Cells Patterning Approach for Human Promyelocytic Leukemia Cells

摘要

To get the detailed information of single cells and screen drugs effectively,especially to develop anti-cancer drugs in view of the cellular heterogeneity of tumors,biochips on single-cell level are necessary.Based on the fabrication techniques of MEMS,substrates with areas of hydrophilicity and hydrophobicity were fabricated to pattern single cells.The optimal process parameters for substrates fabrication are obtained through a series of experiments.MEMS substrates with clear binary surface patterns of hydrophilicity and hydrophobicity are achieved and a general streptavidin template with high bio-compatibility was prepared.In the experiment,biotin-BSA was employed as adhesive proteins Followed by streptavidin and biotinylated antibodies.An array of single HL-60 (Human Promyelocytic Leukemia Cells,HL-60) cells was obtained based on above protein adsorption substrate.The factors affecting the result of the single-cells patterning were investigated and optimized,by which a relatively high patterning rate is achieved.To keep cells characteristics and also for the future parallel patterning of different cells,specific antibodies are investigated for the cell HL-60,including CD45 and CD15.The result of antibodies immobilization and patterning are presented,which can well match the designed template.