Determination of Amoxicillin with Novel Rhodanine Ramification by Flow Injection Chemiluminescence

摘要

3-(4'-fluorophenyl)-5-(2'-sulfonylphenylazo)-rhodanine (4FRASP) firstly synthesized by the authors was used as chemiluminescence (CL) reagent. In acidic medium, CL was observed when amoxicillin was injected into the mixture of 4FRASP-potassium permanganate. Based on this phenomenon, a flow injection CL method was established for the determination of amoxicillin. The CL intensity was correlated linear with the concentration of amoxicillin over the range of 5.0×10-8 to 2.0×10-5 g.mL-1 and the detection limit was 4.5×10-9 g.mL-1.The relative standard deviation (RSD) was 1.3% for determination of 1.0×10-6 g.mL-1 amoxicillin (n=11). This method was applied to the determination of amoxicillin in amoxicillin capsules successfully.