Development of maker-free insect-resistant transgenic rice by Agrobacterium-mediated co-transformation

摘要

＜正＞Agrobacterium-mediated co-transformation is an efficient strategy to generate marker-free transgenic plants.A vector p1300Z-1C containing a synthetic Cry1C gene driven by maize ubiquitin promoter and pCAMBIA1301 harbouring hygromycin phosphotransferase gene（hpt） were introduced into Minghui 86（Oryza sativa L）,an elite indica restorer line.Two independent transformants containing both the Cry1C