Tandem DNA Elements Can Improve Protein Synthesis Efficiency

摘要

Tandem DNA elements, in particular nucleic acid constructs, capable of improving protein synthesis efficiency are IRES enhancers (eg, ScBOI1, ScFLO8, ScNCE102, ScMSN1, K1FLO8, K1NCE102, K1MSN1, K1BOI1) derived from tandem eukaryotic cells (eg yeast). , Ω sequence and yeast-specific Kozak sequence. The use of nucleic acid constructs in yeast-based in vitro biosynthetic systems (eg, yeast-based in vitro protein synthesis systems) can significantly improve protein synthesis efficiency.