Tandem DNA element that can increase protein synthesis efficiency

摘要

A tandem DNA element capable of increasing the efficiency of protein synthesis is provided. In particular, nucleic acid constructs are tandem with IRES enhancers derived from eukaryotic cells (such as yeast) (ScBOI1, ScFLO8, ScNCE102, ScMSN1, KlFLO8, KlNCE102, KlMSN1, K1BOI1, etc.), Ω sequences, and yeast-specific Kozak sequences. It is formed. The use of nucleic acid constructs in yeast-based in vitro biosynthesis systems (eg, yeast-based in vitro protein synthesis systems) can significantly improve protein synthesis efficiency. [Selection diagram] None