METHODS AND REAGENTS FOR HIGH-THROUGHPUT TRANSCRIPTOME SEQUENCING FOR DRUG SCREENING

摘要

Provided is a reagent and method for high-throughput transcriptome in drug discovery and repositioning, which provides a fast and convenient method for drug screening. One step RT-PCR can not only simplify the experimental procedure but also avoid RNA contamination in the experimental process. Furthermore, the reagent and consumable used in the method is cheap and easy to obtain, therefore it can be carried out in ordinary laboratories. Taken together, this method can make drug screening easier and cheaper. Besides, one step RT-PCR method can be applied to high-throughput transcriptome sequencing, which facilitate the wide application of high-throughput transcriptome sequencing.