首页> 外国专利> GENE EXPRESSION CASSETTE FOR EXPRESSING N-TERMINAL METHIONINE-TRUNCATED PROTEIN OF INTEREST AND METHOD FOR PRODUCING N-TERMINAL METHIONINE-TRUNCATED PROTEIN OF INTEREST BY USING SAME

GENE EXPRESSION CASSETTE FOR EXPRESSING N-TERMINAL METHIONINE-TRUNCATED PROTEIN OF INTEREST AND METHOD FOR PRODUCING N-TERMINAL METHIONINE-TRUNCATED PROTEIN OF INTEREST BY USING SAME

机译:用于表达感兴趣的N-末端蛋氨酸截短蛋白的基因表达盒和使用其产生感兴趣的N-末端蛋氨酸截短蛋白的方法

摘要

Disclosed are a gene expression cassette for expressing an N-terminal-methionine-truncated target protein comprising a nucleic acid encoding a target protein and a nucleic acid encoding a cysteine protease, in which a cysteine protease recognition sequence is inserted between methionine (Met), which is the first amino acid at the N-terminus of the target protein, and the second amino acid and N-terminal methionine is cleaved with the cysteine protease, and a method of producing an N-terminal-methionine-truncated target protein using the same.
机译:本发明公开了一种基因表达盒,用于表达N-末端蛋氨酸截短的目标蛋白,其包含编码目标蛋白的核酸和编码半胱氨酸蛋白酶的核酸,其中半胱氨酸蛋白酶识别序列插入在蛋氨酸(Met)之间,蛋氨酸(Met)是目标蛋白N-末端的第一个氨基酸,用半胱氨酸蛋白酶切割第二氨基酸和N端蛋氨酸,以及使用该半胱氨酸蛋白酶生产N端蛋氨酸截短的靶蛋白的方法。

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