APPLICATION OF TOMATO HYDROXYPROLINE-RICH SYSTEMIN PRECURSOR PROTEIN GENE SLHYPSYS IN IMPROVING RESISTANCE OF PLANTS TO VERTICILLIUM WILT

摘要

Provided is an application of the tomato hydroxyproline-rich systemin precursor protein gene SlHypSys in improving the resistance of plants to Verticillium wilt. By means of cloning the tomato hydroxyproline-rich phylogenetic precursor protein gene, SlHypSys, and using molecular biology methods to construct a plant expression vector for constitutive expression of SlHypSys, then using genetic engineering methods to introduce the SlHypSys gene into a plant, transgenic Arabidopsis thaliana, tobacco, and cotton strains having normal transcriptional expression are obtained; the disease indices of all of the obtained transgenic plants are significantly lower than those of the wild-type control, resistance to Verticillium wilt is significantly higher, clearly indicating that the SlHypSys gene can be used for improving the resistance of plants to Verticillium wilt.