Genetic testing chip, its testing method, manufacturing method and microfluidic chip system

摘要

The present invention discloses a genetic test chip, a test method thereof, a manufacturing method thereof, and a microfluidics chip system. Specifically, the sample inlet, the micro passage, the reaction cell, and the sample outlet constitute a genetic inspection passage. When performing a gene test, after completely quenching the fluorescence of the fluorescent label, the sample solution is introduced into the reaction cell from the sample inlet, and when the target gene is present, the target gene is hybridized with the aptamer on the surface of the reaction cell. Cause binding and fluorescence is restored. On the other hand, when the target gene is absent, the fluorescence is still in the quenched state. Therefore, even if the gene test chip does not need to label the target gene with a fluorescent label, the gene test can be realized, the gene test process can be simplified, and the gene molecule not hybridized in the test result can be obtained. It is possible to prevent the influence of the fluorescence signal of the above on the hybridized gene. In addition, the genetic test chip of the present embodiment has a simple structure, is easy to operate, has high aptamer specificity, and has accurate test results.