Type I-B CRISPR-Cas system gene Cas3-based gene editing method

摘要

A novel gene editing system developed based on the gene cas3 in the type I-B CRISPR-Cas system in the chromosome of Streptomyces virginiae IBL14 enables the gene editing of a type I CRISPR-Cas system on biological cell genomes for the first time. In the system, Cas3 can specifically cleave a DNA fragment of interest guided by crRNA or t-DNA. The new approach can be applied to rapid, simple and correct genome editing of prokaryotic and eukaryotic cells, which provides novel supplements and selections for the commercialized gene editing system developed based on Cas9 in type II.