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GERANYLGERANYL PYROPHOSPHATE SYNTHASES

机译:香叶基香叶基焦磷酸合成酶

摘要

The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
机译:本发明涉及一种具有香叶基香叶基焦磷酸合酶活性的变体多肽,该变体多肽包含一个氨基酸序列,当与一个香叶基香叶基焦磷酸合酶对齐时,该氨基酸序列包含一个氨基酸残基的至少一个取代,该氨基酸残基对应于位置92处的任何氨基酸,100或235所述位置参照序号1定义,其中所述变体与具有香叶基基焦磷酸合酶活性的参考多肽相比具有一个或多个修饰性质。本发明的变体多肽可在重组宿主中用于生产甜菊醇或甜菊糖苷。

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