首页> 外国专利> METHOD FOR DETECTING AND QUANTIFYING β-1,6-BRANCHED β-1,3-GLUCAN OR β-1,3-GLUCAN, AND KIT FOR DETECTING AND QUANTIFYING SAME

METHOD FOR DETECTING AND QUANTIFYING β-1,6-BRANCHED β-1,3-GLUCAN OR β-1,3-GLUCAN, AND KIT FOR DETECTING AND QUANTIFYING SAME

机译:检测和量化β-1,6-支化β-1,3-葡聚糖或β-1,3-葡聚糖的方法,以及检测和量化其的试剂盒

摘要

According to the present invention: in a first step, when β-1,6-branched β-1,3-glucan is contained in a test specimen, an active reporter protein is formed with one or the other of split reporter proteins by binding the β-1,6-branched β-1,3-glucan to a first fusion protein and a second fusion protein, and when β-1,3-glucan is contained in a test specimen, an active reporter protein is formed with one or the other of split reporter proteins by binding the β-1,3-glucan to the first fusion protein and a third fusion protein; and in a second step, the active reporter protein formed in the first step is detected and quantified.
机译:根据本发明:在第一步中,当试样中含有β-1,6-支化β-1,3-葡聚糖时,通过将β-1,6-支化β-1,3-葡聚糖与第一融合蛋白和第二融合蛋白结合,并当试样中含有β-1,3-葡聚糖时,由一种或另一种裂解的报告蛋白形成活性报告蛋白,通过将β-1,3-葡聚糖与第一融合蛋白和第三融合蛋白结合,由一种或另一种分裂的报告蛋白形成活性报告蛋白;在第二步中,检测并量化在第一步中形成的活性报告蛋白。

著录项

  • 公开/公告号WO2022050316A1

    专利类型

  • 公开/公告日2022-03-10

    原文格式PDF

  • 申请/专利权人 TOEI SHINYAKU CO. LTD.;

    申请/专利号WO2021JP32154

  • 发明设计人 YAMANAKA DAISUKE;ADACHI YOSHIYUKI;

    申请日2021-09-01

  • 分类号C12Q1/6897;C12N15/12;C12N15/31;C12N15/62;

  • 国家 JP

  • 入库时间 2022-08-24 23:51:11

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