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METHODS FOR IMPROVED MICROSPORE EMBRYOGENESIS AND PRODUCTION OF DOUBLED HAPLOID MICROSPORE-DERIVED EMBRYOS
METHODS FOR IMPROVED MICROSPORE EMBRYOGENESIS AND PRODUCTION OF DOUBLED HAPLOID MICROSPORE-DERIVED EMBRYOS
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机译:改良小孢子胚胎发生和产生双倍单倍体小孢子衍生胚胎的方法
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摘要
The invention provides novel methods for microspore embryogenesis and the production of doubled haploid embryos. For example, the methods provided include obtaining a plurality of flower buds from a donor plant, determining the developmental stage of microspores comprised within said flower buds, selecting flower buds comprising microspores at a desired developmental stage, treating said flower buds, isolating microspores from said flower buds, and culturing said microspores in induction medium or treating said isolated microspores with a chromosome doubling agent.
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