Method for haploid and doubled haploid plant production from embryos obtained by shed microspore culture of hot pepper

摘要

The present invention is directed to a method for producing plants by increasing the re-differentiation rate by 2-step re-differentiation from a pear obtained by culturing pepper shed vesicles. The method for producing plants by 2-step re-differentiation according to the present invention is re-differentiated by reliably inducing the development of cotyledon and normal development of the main lobe by 2-step re-differentiation, which is transferred to a re-differentiation medium and then transferred to a new re-differentiation medium and cultured. It has an effect of increasing the rate, and relates to a method for mass-producing hemiploid and douploid derived from vesicles.