Method for haploid and doubled haploid plant production from embryos obtained by shed microspore culture of hot pepper

摘要

The present invention relates to a method for producing a plant by increasing the regeneration rate by 2-step re-differentiation from embryos obtained by cultivation of red pepper shed vesicles. The method of producing a plant by 2-step re-differentiation according to the present invention is re-differentiated by reliably inducing the development of cotyledons and normal development of the main leaf by 2-step re-differentiation, which is transferred to a re-differentiation medium and then transferred to a new re-differentiation medium and cultured. It has the effect of increasing the rate, and relates to a method for mass-producing vesicle-derived haploids and doubling haploids therefrom.