METHODS ENABLING INFECTION AND DIFFERENTIATION OF HUMAN DISTAL LUNG ORGANOIDS BY SARS-COV-2 AND OTHER PATHOGENS

摘要

We describe a robust human adult distal lung organoid method with a procedure for everting organoids to essentially turn them inside out. This then relocates the apical ACE2-expressing surfaces of cells to the organoid exterior, where they can then be easily infected by SARS-CoV-2 added to the tissue culture medium. Further, this method can be used for infection of any distal lung pathogen that infects apically. Alternatively, if a pathogen interacts basolaterally then eversion is not necessary, and the human adult distal lung organoids can be infected as is.