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MULTIPLE SPECIFIC/NONSPECIFIC PRIMERS FOR PCR OF A COMPLEX GENE POOL
MULTIPLE SPECIFIC/NONSPECIFIC PRIMERS FOR PCR OF A COMPLEX GENE POOL
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机译:用于复合基因库的PCR多种特异性/非特异性引物
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摘要
Disclosed are compositions and methods for single-step PCR of a sample containing a complex target gene pool that can simultaneously amplify a wide variety of variant target gene sequences common to the sample while maintaining the original ratios of gene variants. The compositions and methods described herein utilize (1) a gene-specific primer pool that contains multiple variants that occur in a sample containing a complex mixture of target sequences that are both required for amplification of variants in the mixture which may introduce amplification bias, with (2) a non-specific PCR primer that is designed to target multiple gene-specific primer variants and eliminate amplification bias.
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