A kit and method for identifying the species of Eospalax

摘要

The present invention provides a kit for identifying the genus Protozoa, the kit comprising reagents for detecting the following 14 SNP sites of a species to be detected: a conserved motif sequence shown in SEQ ID NO: 5 in the 12S rRNA gene position 37, position 18 of the conserved motif sequence shown in SEQ ID NO: 6, position 33 of the conserved motif sequence shown in SEQ ID NO: 7, position 7 of the conserved motif sequence shown in SEQ ID NO: 8, SEQ ID NO: ID NO: position 47 of the conserved motif sequence shown in 11, position 1, position 2 of the conserved motif sequence shown in SEQ ID NO: 13, position 4 of the conserved motif sequence shown in SEQ ID NO: 14, SEQ ID NO: position 25 of the conserved motif sequence indicated by 15; In the 16S rRNA gene, position 33 of the conserved motif sequence shown in SEQ ID NO: 9, position 34 of the conserved motif sequence shown in SEQ ID NO: 10, position 35 of the conserved motif sequence shown in SEQ ID NO: 12, SEQ ID NO: ID NO: position 22, position 31 of the conserved motif sequence indicated by 16. The present invention can easily and accurately identify an individual in the genus Protozoa as a species, solves the difficult problem of morphologically identifying a species in the genus Protozoa, and has excellent application prospects.