SIMULTANEOUS DIAGNOSIS METHOD AND KIT FOR SARS-COV-2 INDUCING CORONAVIRUS DISEASE-19 AND SARBECOVIRUS USING PNA PROBE

摘要

The present invention relates to a method for detecting Sarbecovirus which is one of the subgenera of betacoronavirus and novel coronavirus (severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) which induces coronavirus disease-19, by means of a reverse transcription real-time polymerase chain reaction and, more particularly, to a PNA probe and primer for simultaneously detecting Sarbecovirus and novel coronavirus, and a method for diagnosing an infection by using same. The present invention exhibits higher sensitivity and specificity compared to an existing PCR and RT-PCR method using a DNA probe for coronavirus disease-19 diagnosis, and can amplify and distinguish ORF1ab gene, S gene and N gene of the novel coronavirus and E gene of Sarbecovirus, and thus can analyze an infection of the novel coronavirus and Sarbecovirus with high sensitivity and accuracy.