PIG WHOLE GENOME SGRNA LIBRARY, AND CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF

摘要

A pig genome-wide sgRNA library, a method for constructing the same, and a use thereof. The library includes sgRNA sequences of 20438 genes in a pig (Sus scrofus) whole genome, of which 17410 genes are designed to obtain 6 sgRNAs and 2828 genes are designed to obtain 1-5 sgRNAs. The sgRNAs are acquired by the following process: acquiring candidate sgRNAs from a target sequence, performing an off-target analysis on and scoring the candidate sgRNAs, and finally filtering sgRNAs according to a scoring result. Details and process in modules are optimized by using multiple design criteria and screening principles and finally the pig genome-wide sgRNA library is constructed.