A method of pretreatment of transparency of a biological samples with a size of 1 mm or less and a method of transparency of the biological samples comprising the same

摘要

According to the present invention, the pretreatment method for clearing a biological sample with a size of 1 mm or less, that is, spheroids or organoids, uses phosphate-buffered saline (PBS), not a conventional sucrose solution, as a pretreatment solution. Therefore, in the pre-treatment process, the problem of spheroids or organoids floating on the surface of the water due to the difference in density and damage to the sample structure was solved, and transparency was possible while maintaining the original shape of the spheroids or organoids, so the effect of imaging deep have.