A METHOD FOR DETERMINING THE SEVERITY OR GRADE OF HUMAN PAPILLOMAVIRUS (HPV)-INDUCED DYSPLASIA

摘要

The invention relates to an in vitro method for determining the severity or a grade of a human papillomavirus (HPV)-induced dysplasia or whether cervical carcinoma is present, and related materials, devices and computer-implementation of the method. The present invention comprises quantitatively determining an expression level of (i) viral and (ii) cellular messenger RNA (m RNA) in a sample obtained from the subject, wherein the determined viral m RNA encodes an HPV oncoprotein E6 and/or E7, and wherein the determined cellular m RNA comprises m RNA of at least one cellular proliferation marker, of at least one cancer stem cell marker, and of at least one tumor marker, and deducing from the quantity of said viral m RNA and said cellular m RNA the severity or a grade of the dysplasia or whether cervical carcinoma is present in the subject.