Transient Expression of Human Papillomavirus Type 16(HPV 16) mRNA in Normal Human Keratinocytes Transfected by pSV2-neo/16

摘要

Objective:To investigate the expression of HPV16 mRNA in normal human keratinocytes transfected with pSV2-neo/16.First human keratinocytes were cultured in the serum-free medium M154.Second,the plasmid pSV2-neo/16 was transfected into the human keratinocytes using a transfecting reagent.Third,RT-PCR and Southern Blotting were used to detect the expression of HPV16 mRNA and DNA in the transfected keratinocytes,respectively.Results:The expression of HPV 16 mRNA was successfully amplified and an 110bp was ditected by RT-PCR.A 7.9kb fragment was confirmed in the transfected keratinocytes by Southern Blot analysis.Conclusion:HPV 16 mRNA and DNA were successfully detected in the human keratinocytes.