PCR Method for detecting maritime pathogenic bacteria using two-step PCR and primer set composition and kit for the same

摘要

The present invention relates to a method for detecting marine pathogenic bacteria using two-step PCR, and to a primer set, composition and kit for the same, specifically, a) isolating DNA from a sample; b) using the isolated DNA as a template, a primer set consisting of a forward primer consisting of the sequence of SEQ ID NO: 1 and a reverse primer consisting of the sequence of SEQ ID NO: 2; A primer set consisting of a forward primer consisting of the sequence of SEQ ID NO: 3 and a reverse primer consisting of the sequence of SEQ ID NO: 4; And a primer set consisting of a forward primer consisting of the sequence of SEQ ID NO: 5 and a reverse primer consisting of the sequence of SEQ ID NO: 6; performing a two-step polymerase chain reaction using a primer set selected from among; and c) detecting an amplification product including the forward primer and the reverse primer from among the amplification products generated by the polymerase chain reaction; it's about