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Target sequence Nucleic acid detection method, virus detection method, and virus detection kit

机译:靶序列核酸检测方法,病毒检测方法和病毒检测试剂盒

摘要

PROBLEM TO BE SOLVED: To provide a detection method capable of detecting a virus easily and at low cost in a short time. A method for detecting a target sequence nucleic acid in a genome, wherein the amplification step of amplifying the target sequence nucleic acid using the target sequence nucleic acid in the genome as a template sequence and the target sequence nucleic acid amplified by the amplification step are used. The detection step comprises a detection step of electrochemically detecting, in the presence of an oxidation-reducing substance that binds to a complementaryly binding double-stranded portion of the amplification product of the target sequence nucleic acid amplified in the amplification step. A method for detecting a target sequence nucleic acid, which is carried out by applying to the working electrode and the counter electrode and measuring the current between the two electrodes. [Selection diagram] Fig. 2
机译:要解决的问题:提供一种能够在短时间内容易地检测病毒的检测方法。 使用用于在基因组中检测靶序列核酸的方法,其中使用基因组中的靶序列核酸作为模板序列和通过扩增步骤扩增的靶序列核酸来扩增步骤 。 检测步骤包括在氧化还原物存在下电化学检测的检测步骤,其结合在扩增步骤中扩增的靶序列核酸的扩增产物的互补的结合产物的互补的双链部分。 一种检测靶序列核酸的方法,通过施加到工作电极和对电极并测量两个电极之间的电流来进行。 [选择图]图2

著录项

  • 公开/公告号JP2021185812A

    专利类型

  • 公开/公告日2021-12-13

    原文格式PDF

  • 申请/专利权人 BIOSEEDS株式会社;

    申请/专利号JP20200093786

  • 发明设计人 マニシュ ビヤニ;

    申请日2020-05-28

  • 分类号C12Q1/6825;C12Q1/6844;C12Q1/70;C12M1/34;C12Q1/6876;G01N33/50;G01N33/53;G01N33/569;C12N15/10;

  • 国家 JP

  • 入库时间 2022-08-24 22:47:08

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