Method of forming lipid bilayer membrane and partition wall and equipment for that purpose

摘要

PROBLEM TO BE SOLVED: To reduce the amount of lipid used for forming a lipid bilayer film as compared with a known droplet contact method, and to form a lipid bilayer film with better reproducibility and in a short time. Further, the present invention provides a novel method for forming a lipid bilayer membrane, a novel partition wall used therein, and a lipid bilayer membrane forming apparatus capable of reducing the amount of proteins and the like retained in the lipid bilayer membrane. matter. SOLUTION: The method for forming a lipid bilayer film is a partition wall having a through hole in which a lipid bilayer film is formed, and a lipid film-forming lipid is formed in the slit of the partition wall in which a slit is formed in the inner wall of the through hole. A step of supplying a lipid liquid containing the above to expose the lipid liquid to the inner wall surface, and a step of adding a first aqueous solution to one side separated by a partition wall to form a lipid bilayer in the through hole, and a step separated by the partition wall. It comprises a step of adding a second aqueous solution to the other side to form a lipid bilayer in the through hole. [Selection diagram] FIG. 6