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IMMUNOCHROMATOGRAPHIC TEST KIT FOR EXTRACTING AND MEASURING SUGAR CHAIN ANTIGENS AND CAPABLE OF CONTROLLING ANALYTE DEVELOPMENT
IMMUNOCHROMATOGRAPHIC TEST KIT FOR EXTRACTING AND MEASURING SUGAR CHAIN ANTIGENS AND CAPABLE OF CONTROLLING ANALYTE DEVELOPMENT
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机译:免疫层析试剂盒提取和测量糖链抗原,能够控制分析物发育
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摘要
The present invention aims to provide: an immunochromatographic test kit for controlling the development of a specimen on an immunochromatographic test strip and for appropriately controlling a treatment using an acid reagent, a nitrite and a neutralizing reagent; and an immunochromatography method using the kit. The immunochromatographic test kit includes: (i) a nitrite solution or an acid solution; and (ii) an immunochromatographic test strip for extracting and measuring a sugar chain antigen in a specimen, comprising: a sample pad to which a specimen mixed with the nitrite or acid solution is added; a label region comprising a labeled antibody obtained by labeling an antibody against the sugar chain antigen; and a detection region on which an antibody against the sugar chain antigen is immobilized, wherein an antibody-sugar chain antigen-labeled antibody complex is formed in the detection region to measure the sugar chain antigen, and wherein the immunochromatographic test strip has a region impregnated with a neutralizing reagent upstream of the label region, and further has, upstream of the region impregnated with the neutralizing reagent, a region impregnated with a solid acid reagent when the specimen mixed with the nitrite is used, or a region impregnated with the nitrite when the specimen mixed with the acid solution is used, and wherein the nitrite or acid solution comprises one or more selected from the group consisting of polyoxyethylene octyl phenyl ether, a quaternary ammonium compound, phosphoric acid, sodium hydroxide, N-acetyl-L-cysteine, NaCl, PVA, BSA and NaBr.
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