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IMMUNOCHROMATOGRAPHIC TEST KIT FOR EXTRACTING AND MEASURING SUGAR CHAIN ANTIGENS AND CAPABLE OF CONTROLLING ANALYTE DEVELOPMENT
IMMUNOCHROMATOGRAPHIC TEST KIT FOR EXTRACTING AND MEASURING SUGAR CHAIN ANTIGENS AND CAPABLE OF CONTROLLING ANALYTE DEVELOPMENT
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机译:免疫层析测试试剂盒,用于提取和测定糖链抗原并具有控制分析物开发的能力
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摘要
The present invention aims to provide: an immunochromatographic test kit for controlling the development of analytes on an immunochromatographic test piece and for appropriately controlling processing using acidic reagents, nitrite, and neutralizing reagents; and an immunochromatography method using said kit. The immunochromatographic test kit includes an immunochromatographic test piece that includes: a sample pad to which is added an analyte that has mixed therein (i) a nitrite solution or an acidic solution and (ii) nitrite or an acidic solution; a marker region that includes a marker antibody marking an antibody for sugar chain antigens; and a detection region in which the antibody for sugar chain antigens is immobilized. The immunochromatographic test piece forms an antibody–sugar chain antigen–marker antibody complex in the detection region and measures the sugar chain antigens. The immunochromatographic test piece is for extracting and measuring sugar chain antigens in the analyte and has: a region impregnated with a neutralizing reagent, upstream from the marker region; and, upstream from the region impregnated with the neutralizing reagent, a region impregnated with a solid acidic reagent, when an analyte mixed with nitrite is used, or a region impregnated with nitrite, when an analyte mixed with acidic solution is used. The nitrite or acidic solution include at least one selected from the group consisting of polyoxyethylene octyl phenyl ether, a quaternary ammonium compound, phosphoric acid, sodium hydroxide, N-acetyl-L-cysteine, NaCl, PVA, BSA, and NaBr.
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