The present invention relates to a novel Leukonostok sp. strain and a bioconversion method of paoniflorin using the same, and more particularly, to a method for converting paoniflorin into β-gentiobiosyl paeoniflorin. It relates to a Leuconostoc sp. LN180020 strain showing bioconversion ability and a bioconversion method of paoniflorin using the same. Since the LN180020 strain of the leukonostok genus provided by the present invention has very excellent bioconversion ability to convert paoniflorin into β-gentiobiosyl paoniflorin with improved physiological activity, prevention of various diseases including diabetes, obesity, etc. Alternatively, it may be very usefully utilized in the development of therapeutic agents.
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