Flying fish and hot smelt, which are fish species used in various recipes, are continuously reported to be mixed and sold due to some morphological characteristics. As cases are reported not only in the products sold but also in general restaurants, the species identification method for flying fish and hot smelt must be quickly and easily used not only in the laboratory but also in the field. Even if it is, it should be possible to identify it. Therefore, the method for determining flying fish and hot smelt using general PCR and ultra-high-speed real-time PCR developed in the present invention can specifically discriminate only flying fish and hot smelt, which can prevent mixed sales of flying fish and hot smelt, thereby activating the consumer market. It is expected to not only contribute to the
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