Provided is a gene editing system comprising (i) a polynucleotide encoding an endonuclease under the control of an inducible expressing regulatory sequence; (ii) a guide polynucleotide targeting a female-essential genomic sequence that is required for female-specific viability, and (iii) a guide polynucleotide targeting a male sterility genomic sequence that is required for male-specific fertility. Additionally provided are insect eggs, an insects, and an insect populations, each of which is genetically modified by the gene editing system. Further provided are methods and compositions relating to producing such systems, insect eggs, insects, insect populations and uses thereof in reducing a wild-type insect population.
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