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OLIGONUCLEOTIDE-TYRAMIDE CONJUGATE AND USE OF THE SAME IN TYRAMIDE-SIGNAL AMPLIFICATION (TSA)-BASED DETECTION METHODS
OLIGONUCLEOTIDE-TYRAMIDE CONJUGATE AND USE OF THE SAME IN TYRAMIDE-SIGNAL AMPLIFICATION (TSA)-BASED DETECTION METHODS
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机译:寡核苷酸 - 酪胺缀合物和在酪酰胺信号放大(TSA)的检测方法中的使用相同
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摘要
Provided herein, among other things, is an oligonucleotide-tyramide conjugate, which conjugate can be used in variety of tyramide-signal amplification (TSA)-based detection methods. Some embodiments of the method may comprise incubating a sample that has a binding agent-peroxidase conjugate bound to sites thereon (where the peroxidase may directly linked to the binding agent or indirectly directly linked to the binding agent via a pair of oligonucleotides that are hybridized together) with an oligonucleotide-tyramide conjugate in the presence of hydrogen peroxide, thereby activating the tyramide and causing deposition of the oligonucleotide of the oligonucleotide-tyramide conjugate onto sites that are proximal to the peroxidase of the binding agent-peroxidase conjugate.
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