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LARGE VOLUME SAMPLE INJECTION FOR LIQUID CHROMATOGRAPHY
LARGE VOLUME SAMPLE INJECTION FOR LIQUID CHROMATOGRAPHY
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机译:液相色谱大量样品注射液
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摘要
Described are methods and fluidic networks for injecting a sample into a liquid chromatography system. The method may be applied for separations in which increased sensitivity is desired and a large sample volume in a strong solvent is to be injected. The method may include merging a flow of a solvent having a sample dissolved therein with a flow of a diluent. The merging occurs in a fluidic path to an injection valve. The full amount of sample previously contained in a smaller volume of solvent is maintained in a larger volume of a diluted sample that includes the strong solvent and the diluent. The entire volume of the diluted sample is loaded into a sample loop coupled to the injection valve and subsequently injected into the system flow of the chromatography system.
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