Immunochromatographic test kit for extracting and measuring sugar chain antigen, which can control the development of a sample

摘要

The present invention relates to an immunochromatographic test kit for controlling the development of a specimen on an immunochromatographic test piece and for appropriately controlling treatment with an acid reagent, nitrite and neutralizing reagent, and an immunochromatographic method using the kit aims to provide (i) a nitrite solution or an acid solution, and (ii) a sample pad to which a sample mixed with nitrite or acidic solution is added, a label region containing a labeled antibody labeled with an antibody against the sugar chain antigen, and detection by immobilizing the antibody against the sugar chain antigen An immunochromatographic test piece comprising a region and measuring a sugar chain antigen by forming a complex of antibody-sugar chain antigen-labeled antibody in the detection region, comprising a region impregnated with a neutralizing reagent upstream of the label region; Sugar chains in the sample having a region impregnated with a solid acidic reagent upstream of the region impregnated with the neutralizing reagent, when using a sample mixed with nitrite, and having a region impregnated with nitrite when using a sample mixed with an acidic solution An immunochromatographic test kit comprising an immunochromatographic test piece for extracting and measuring an antigen, wherein nitrite or an acidic solution is polyoxyethylene octylphenyl ether, quaternary ammonium compound, phosphoric acid, sodium hydroxide, N-acetyl-L- An immunochromatographic test kit comprising at least one selected from the group consisting of cysteine, NaCl, PVA, BSA, and NaBr.