The present invention provides methods for producing a heme pe- roxidase from inclusion bodies (IBs) comprising the steps of: providing the heme peroxidase in the form of IBs, solubilizing said IBs, transferring said solubilized IBs into a refolding buffer to obtain a refolding mix, adding a heme cofactor to said refolding mix, wherein the addition of the heme cofactor to the refolding mix is distributed over a time period of at least 1 hour. The invention further provides methods for producing heme peroxidase products.
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