Method for analyzing biological samples and method for evaluating mitochondrial function

摘要

PROBLEM TO BE SOLVED: To provide a method for analyzing a biological sample capable of accurately quantifying 2SC in a wide range of biological samples. SOLUTION: This is an analysis method of a biological sample in which S- (2-succinyl) cysteine (2SC) in a biological sample is quantified by using a liquid chromatography-mass analysis method. A pretreatment step including a degreasing step of performing a degreasing treatment, a precipitation step of precipitating a protein or a fragment thereof and recovering a precipitate containing the 2SC-ized protein or a fragment thereof, and a hydrolysis step of hydrolyzing with hydrochloric acid in this order. A method for analyzing a biological sample, which comprises performing. [Selection diagram] None