Methods to analyze the degradation of cellulose in the hydrolysis of cellulosic material in biomass hydrolysis, and hydrolysis of lignocellulosics, method for determining whether an enzyme of interest affects the hydrolysis of cellulose, the method of high productivity to analyse the enzymes and / or polipetu00ed Buildings of interest, method for analyzing the performance of enzyme system to evaluate the performance of the enzyme.Method for normalizing methods to determine the amount of cellulose, the target of a biological sample, method to normalize the fluorescent intensity data, and method for determining whether an enzyme of interest and a polypeptide of interest affect the hydrolysis of cellulose

摘要

Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of, inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed.