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Separation of enzymes from Trichoderma reesei by filter press and ceramic membrane tangential filtration
Separation of enzymes from Trichoderma reesei by filter press and ceramic membrane tangential filtration
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机译:通过过滤压机和陶瓷膜切向过滤从Trichoderma Reesei分离酶
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摘要
The invention relates to a method for separating, from a culture medium, an enzymatic cocktail and the fungus Trichoderma reesei, the culture medium resulting from enzyme production by the fungus, method in which: said culture medium is subjected, in a period of no longer than 30 h from the halting of production, to a separation on a filter press lined with a fabric having a porosity of 3-20 μm, so as to obtain a filtrate having a corrected optical density OD at 600 nm of less than 2.5; and the liquid phase obtained is subjected to tangenital micro-filtration on a ceramic membrane having a cut-off limit of 0.5 to 1.4 μm, so that the corrected OD at 600 nm does not exceed 0.1.
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