Bioconjugate Vaccine Synthesis in Prokaryotic Cell Lysates

摘要

Disclosed are methods, systems, components and compositions for the cell-free synthesis of glycosylated proteins that can be used in vaccines, including anti-bacterial vaccines. A glycosylated protein may comprise a bacterial polysaccharide conjugated to a carrier, which may be used to generate an immune response in a host immunized against the polysaccharide conjugated to the carrier. Suitable carriers include Haemophilus influenzae protein D (PD), Neisseria meningitidis porin protein (PorA), Corynebacterium diphtheriae toxin (CRM197), Clostridium tetani toxin (TT), and Escherichia coli maltose. binding proteins, and variants thereof.