A combination of chemical and genetic approaches to produce artificial pluripotent stem cells

摘要

Problem to be solved: to provide a method for identifying and using small molecules to induce pluripotency in mammalian cells and to induce pluripotency.Polynucleotides encoding OCT polypeptidesA polynucleotide encoding KLF polypeptideOne or more expression cassettes containing polynucleotides encoding the Sox polypeptide;Exogenous OCT polypeptideExogenous Sox polypeptideIntroducing an exogenous KLF polypeptide into the mammalian non pluripotent cell;Mammalian mammalian non pluripotent cellsGSK3 inhibitorMEK inhibitorAnd TGF β Including the step of contacting the receptor / ALK5 inhibitorMethod.Diagram