首页> 外国专利> METHOD FOR PRODUCING DEXTRAN POLYMER-BASED, AMPLIFIED NUCLEIC ACID APTAMER NANOCONSTRUCT SELECTIVELY CAPTURING TARGET MOLECULE

METHOD FOR PRODUCING DEXTRAN POLYMER-BASED, AMPLIFIED NUCLEIC ACID APTAMER NANOCONSTRUCT SELECTIVELY CAPTURING TARGET MOLECULE

机译:制备葡聚糖基于聚合物的扩增核酸适体纳米核结构的靶线捕获靶分子

摘要

The present invention relates to a method for producing dextran polymer-based amplified nucleic acid aptamer nanoconstructs which efficiently and selectively capture a specific target molecule, the method comprising linking a short nucleic acid sequence or a complementary sequence for formation of nanoconstructs and a nucleic acid aptamer sequence for capture of the specific target molecule to a dextran polymer by a chemical reaction, mixing the resulting polymer/nucleic acid substances with each other to form nanostructures, subjecting the nanostructures to rolling circle amplification, thereby forming a nucleic acid aptamer having a repeated structure. The dextran polymer-based amplified nucleic acid aptamer nanoconstructs have the effect of efficiently and selectively capturing a specific target molecule.
机译:本发明涉及生产基于葡聚糖聚合物的扩增核酸适体纳米结构的方法,其有效且选择性地捕获特定的靶分子,该方法包括连接短核酸序列或用于形成纳米结构和核酸适体的互补序列 通过化学反应将特定靶分子捕获到葡聚糖聚合物的序列,将所得聚合物/核酸物质彼此混合形成纳米结构,使纳米结构进行滚动圆扩增,从而形成具有重复结构的核酸适体 。 葡聚糖聚合物的扩增核酸适体纳米结构具有有效和选择性地捕获特异性靶分子的效果。

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