Vesicles for unscavenging delivery of guide RNA molecules and / or guide RNA molecules / RNA induced nuclease complexes and their production methods

摘要

The present invention describes a crisp related RNA inducible nuclease ribonucleoprotein complex (RNP) efficiently delivered to the target cell by vesicles possessing gRNA and / or fusion envelope proteins.The present invention discloses a method for producing a vesicle loaded with a gRNA complexed with a gRNA or nuclease and the vesicle production.The cytoplasmic transcription system is used to accumulate gRNA in the cytoplasm to achieve highly efficient incorporation of gRNA into the vesicles.Such a cytoplasmic transcription system may be operated only in an acceptable cell.