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Recombinant cell comprising a gene encoding a protein of interest in hotspot and preparation method and use thereof
Recombinant cell comprising a gene encoding a protein of interest in hotspot and preparation method and use thereof
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机译:重组细胞,包括编码热点的感兴趣蛋白质的基因及其制备方法和用途
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摘要
It is related to the technology of inserting and expressing the coding gene for a foreign target protein at a specific position in the genome of an animal cell. More specifically, it relates to a recombinant cell in which one or more target protein-encoding genes are inserted into one or more hot spots in the genome of an animal cell, a method for producing the recombinant cell, and a method for producing a target protein comprising the step of culturing the recombinant cell will be. Provided herein is a technology capable of stably (continuously) and highly efficient production of a recombinant protein by inserting and culturing a coding gene for an exogenous recombinant protein in a hotspot in the genome of a eukaryotic host cell provided herein. This technique can be utilized to secure a large amount of various recombinant proteins. In addition, two or more different types of proteins can be simultaneously produced from one cell by inserting and expressing the coding genes of two or more different recombinant proteins into different hotspots in the same host cell.
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