An improved method for genome editing and programmable nuclease free genome editing with programmable nucleases

摘要

Problem to be solved: to provide a composition and a method for genome editing in an isolated cell or organism.Steps for introducing an editing oligonucleotide into cells or organisms without additional exogenous proteins or nucleic acids for assisting the editing of the target nucleic acid basesThe edited oligonucleotide comprises one or more modificationsThe modification may be one or more skeletal modificationsSugar modificationAnd / or nucleic acid base modificationThe edited oligonucleotide is substantially complementary to the genomic sequence containing the target nucleic acid base;The modification of the edited oligonucleotide isIt increases the efficiency of editingThe site directed deamination of the target nucleic acid base is deamination of the cytosine nucleic acid base to the uracil nucleic acid base induced by crisprna of the edited oligonucleotide and the inactive cas9Method.No selection