Method for converting genome sequence of monocotyledons specific to target DNA sequence and transforming nucleic acid base and molecular complex used for it

摘要

The present invention relates to a method for modifying a targeted site of a double-stranded DNA possessed by a monocotyledonous plant cell, which specifically binds to a target nucleotide sequence in the selected double-stranded DNA. And the nucleobase converting enzyme-bound complex is brought into contact with the double-stranded DNA, and the targeted ligation is performed without cleaving at least one strand of the double-stranded DNA at the targeted site. Converting or deleting one or more nucleotides of the selected site into another one or more nucleotides, or inserting one or more nucleotides into the targeted site, the double-stranded DNA and the complex Is contacted by introduction of a nucleic acid encoding the complex into the monocotyledonous plant cell. Furthermore, a complex in which a nucleic acid sequence recognition module that specifically binds to a target nucleotide sequence in double-stranded DNA of a monocotyledonous plant cell and a nucleobase converting enzyme bound for use in the method is also provided. .