A method for converting monocot genome sequences in which a nucleic acid base in a targeting DNA sequence is specifically converted, and a molecular complex used therein.

摘要

The present invention provides a method of modifying a target region of double-stranded DNA of monocotyledonous cells, comprising a complex in which a nucleic acid sequence-recognition module and a nucleic acid nucleotide converting enzyme are specifically bound to a target nucleotide sequence in a predetermined double-stranded DNA. Contacting the double stranded DNA to convert one or more nucleotides to another one or more nucleotides or delete one or more nucleotides at the target site without cutting at least one chain of the double stranded DNA at the target site Inserting a nucleotide into the target site, wherein the double-stranded DNA contains the nucleic acid encoding the complex into the monocotyledonous cell and cultures the monocotyledonous cell to express the complex intracellularly. In contact with the invention. In addition, there is also provided a complex used in the above method, wherein a nucleic acid sequence-recognition module and nucleic acid base converting enzyme that specifically bind to a target nucleotide sequence in the double stranded DNA of the monocotyledonous cell are bound.