Method for Purifying Heterodimer Multispecific Antibody from Parent Homo Dimer Antibody Species

摘要

PROBLEM TO BE SOLVED: To provide a multispecific antibody (MAI) for the purpose of co-binding to at least two different antigens or epitopes (also referred to as "target" and used interchangeably throughout) by MAI and a parent homodimer antibody species. To provide a method of purifying from the composition contained. Reagents that can be used to carry out such methods are also provided. The present invention is particularly advantageous for at least two different antigens or epitopes, including a first polypeptide comprising a first heavy chain (HC) variable region and a heterodimer comprising a second polypeptide comprising a second HC variable region. Provided is a method of purifying a multispecific antibody (MAI) of interest to co-bind with a composition comprising MAI and at least two corresponding parent homodimer antibody species. [Selection diagram] None