Method for purifying heterodimeric multispecific antibodies from parent homodimeric antibody species

摘要

A composition comprising a multispecific antibody of interest (MAI) co-binding to at least two different antigens or epitopes (also referred to as “target” and used interchangeably throughout), MAI and a parent homodimeric antibody species Also provided are methods of purifying from products and reagents that can be used to carry out such methods. The invention is particularly advantageous for at least two different antigens or epitopes comprising a heterodimer comprising a first polypeptide comprising a first heavy chain (HC) variable region and a second polypeptide comprising a second HC variable region. A method of purifying a multispecific antibody of interest (MAI) that co-couples to a composition comprising MAI and at least two corresponding parent homodimeric antibody species is provided. [Selection] Figure 2